Identification of protein aggregates in the aging vertebrate brain with prion-like and phase separation properties
Itamar Harel , Yiwen R. Chen, Inbal Ziv, Param Priya Singh, Paloma Navarro Negredo, Uri Goshtchevsky, Wei Wang, Gwendoline Astre, Eitan Moses, Andrew McKay, Ben E. Machado, Katja Hebestreit, Sifei Yin, View Alejandro Sánchez Alvarado, Daniel F. Jarosz, Anne Brunet
BioRxiv
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 166136 | pDONR221-mRuby3 | Gateway entry vector for mRuby3 |
| 166137 | pAG415GPD-EGFP | EGFP expression under the control of the GPD promoter yeast |
| 166138 | pAG415GPD-mRuby3 | mRuby3 expression under control of the GPD promoter in yeast |
| 166139 | pAG416GAL-ccdB-mRuby3 | Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a CEN/ARS element for low copy number when in yeast. |
| 166140 | pAG416GAL-EGFP | EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast. |
| 166141 | pAG416GAL-mRuby3 | mRuby3 expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast. |
| 166142 | pAG426GAL-ccdB-mRuby3 | Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast. |
| 166143 | pAG426GAL-EGFP | EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast. |
| 166144 | pAG426GAL-mRuby3 | mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast. |
| 166145 | pDEST17-His10-ccdB | Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli. |
| 166146 | pDEST17-His10-ccdB-SNAP | Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli. |
| 166147 | pDEST17-His10-Smt3-ccdB | Gateway destination vector for generation of N-terminal His10-Smt3 tagged proteins in E. coli. |
| 166148 | pDEST17-His10-Smt3-ccdB-SNAP | Gateway destination vector for generation of N-terminal His10-Smt3 and C-terminal SNAP tagged proteins in E. coli. |
| 166149 | pDEST17-His10-Smt3-DDX5(kf)-SNAP | Expression of N. furzeri DDX5 in E. coli |
| 166150 | pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP | Expression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coli |
| 166151 | pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP | Expression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coli |
| 166152 | pDEST17-His10-DDX5(kf)-SNAP | Expression of N. furzeri DDX5 in E. coli |