Richard Baker Lab: A structure-based mechanism for initiation of AP-3 coated vesicle formation
Begley M, Aragon M, Baker RW
Proc Natl Acad Sci (PNAS)
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221766 | MZB216 | "Empty" C-terminal CPD-10xHis vector for rapid cloning/DNA assembly via SwaI restriction site for E. coli based protein expression |
| 221767 | MZB215 | Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221769 | MZB218 | Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221770 | MZB219 | Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221771 | MZB220 | Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag |
| 221772 | MZB222 | Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases |