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Syntaxin 17 recruitment to mature autophagosomes is temporally regulated by PI4P accumulation.

Shinoda S, Sakai Y, Matsui T, Uematsu M, Koyama-Honda I, Sakamaki JI, Yamamoto H, Mizushima N
Elife. 2024 Jun 4;12:RP92189. doi: 10.7554/eLife.92189. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221737pMRXIP-GFP-STX17TMΔCStable expression of GFP-STX17TMΔC in mammalian cells
221738pMRXIB-mRuby3-LC3Stable expression of mRuby3-LC3 in mammalian cells
221739pMRXIP-GFP-9K0QStable expression of GFP-9K0Q in mammalian cells to monitor membrane charge
221740pMRXIP-GFP-5K4QStable expression of GFP-5K4Q in mammalian cells to monitor membrane charge
221741pMRXIP-GFP-3K6QStable expression of GFP-3K6Q in mammalian cells to monitor membrane charge
221742pMRXIP-GFP-1K8QStable expression of GFP-1K8Q in mammalian cells to monitor membrane charge
221743pMRXIP-GFP-0K9QStable expression of GFP-0K9Q in mammalian cells to monitor membrane charge
221744pMRXIB-mRuby3-STX17TMStable expression of mRuby3-STX17TM in mammalian cells
221745pMRXIP-GFP-OSBP(PHD)Stable expression of GFP-OSBP(PHD) in mammalian cells
221746pMRXIP-GFP-CERT(PHD)Stable expression of GFP-CERT(PHD)in mammalian cells
221747pMRXIP-GFP-P4M-SidMx2Stable expression of GFP-P4M-SidMx2 in mammalian cells
221748pMRXIP-GFP-FAPP(PHD)Stable expression of GFP-FAPP(PHD) in mammalian cells
221749pMRXIB-WIPI2b-mRuby3Stable expression of WIPI2b-mRuby3 in mammalian cells
221750pMRXIP-GFP-2xFYVEStable expression of GFP-2xFYVE in mammalian cells
221751pMRXIP-GFP-ING2(PHD)Stable expression of GFP-ING2(PHD) in mammalian cells
221752pMRXIP-GFP-TRPML1(PHD)Stable expression of GFP-TRPML1(PHD) in mammalian cells
221753pMRXIP-GFP-PLCd1(PHD)Stable expression of GFP-PLCd1(PHD) in mammalian cells
221754pMRXIP-GFP-Evectin-2Stable expression of Evectin-2 in mammalian cells
221755pMRXIP-GFP-PKD C1abStable expression of GFP-PKD C1ab in mammalian cells
221756pMRXIP-Btk1(PHD)-GFPStable expression of Btk1(PHD)-GFP in mammalian cells
221757pMRXIP-GFP-TAPP1(PHD)Stable expression of GFP-TAPP1(PHD) in mammalian cells
221758pMRXIP-GFP-Spo20(PABD)Stable expression of GFP-Spo20(PABD) in mammalian cells
221759pMRXIP-GFP-PI4KBStable expression of GFP-PI4KB in mammalian cells
221760pMRXIP-GFP-PI4K2AStable expression of GFP-PI4K2A in mammalian cells
221761pMRXIP-GFP-CERT(PHD)(W33A)Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells
221762pMRXIB-mRuby3-CERT(PHD)Stable expression of mRuby3-CERT(PHD) in mammalian cells
222586pMRX-IZU-LAMP1-mRuby3Stable expression of LAMP1-mRuby3 in mammalian cells

Antibodies from Article