Protocol for detecting genomic insulators in Drosophila using insulator-seq, a massively parallel reporter assay.
Tonelli A, Cousin P, Gambetta MC
STAR Protoc. 2024 Oct 24;5(4):103391. doi: 10.1016/j.xpro.2024.103391.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221458 | insulator_seq_barcode_vector | Vector for generating a barcode library for insulator-seq. |
| 221459 | insulator_seq_UENU_donor | Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library. |
| 221460 | insulator_seq_reporter_vector | Vector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library. |