A dual-plasmid CRISPR/Cas9-based method for rapid and efficient genetic disruption in Mycobacterium abscessus.
Neo DM, Clatworthy AE, Hung DT
J Bacteriol. 2024 Mar 21;206(3):e0033523. doi: 10.1128/jb.00335-23. Epub 2024 Feb 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221386 | pDN-Sth1Cas9 | Cas9 under control of AHT inducible TetR on integrating plasmid (KanR) using L5 integrase and attP for site-specific integration into attB |
| 221387 | pDN-Cherry-sgRNA | episomal (HygR), with constitutive mCherry (Psmyc) and AHT-inducible sgRNA cloning site, including Golden Gate for multiple sgRNAs |