pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism.
Landau LM, Chaudhary N, Tien YC, Rogozinska M, Joshi S, Yao C, Crowley J, Hullahalli K, Campbell IW, Waldor MK, Haigis M, Kagan JC
Mol Cell. 2024 Jul 11;84(13):2436-2454.e10. doi: 10.1016/j.molcel.2024.05.030. Epub 2024 Jun 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221239 | pMSCV-FKBP-empty | Expression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction |
| 221240 | pMSCV-FKBP-STING(pLxIS) | Expression of STING CTT in mammalian cells by retroviral transduction |
| 221241 | pMSCV-FKBP-STING(pLxIS) S365A | Expression of mutant STING CTT in mammalian cells by retroviral transduction |
| 221242 | pMSCV-FKBP-STING(pLxIS) L373A | Expression of mutant STING CTT in mammalian cells by retroviral transduction |
| 221243 | pMSCV-FKBP-STING(FL) | Expression of STING without transmembrane domain in mammalian cells by retroviral transduction |
| 221244 | pMSCV-FKBP-TRIF(Nterm) | Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction |
| 221245 | pMSCV-FKBP-TRIF(pLxIS) S209A | Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction |
| 221246 | pMSCV-FKBP-TRIF(pLxIS) L193A | Expression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction |
| 221247 | pMSCV-FKBP-TRIF(trunc 1-23) | Expression of TRIF truncation 1 (24-222) in mammalian cells by retroviral transduction |
| 221248 | pMSCV-FKBP-TRIF(trunc 1-43) | Expression of TRIF truncation 2 (44-222) in mammalian cells by retroviral transduction |
| 221249 | pMSCV-FKBP-TRIF(trunc 1-63) | Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction |
| 221250 | pMSCV-FKBP-TRIF(trunc 1-83) | Expression of TRIF truncation 4 (84-222) in mammalian cells by retroviral transduction |
| 221251 | pMSCV-FKBP-TRIF(trunc 1-103) | Expression of TRIF truncation 5 (104-222) in mammalian cells by retroviral transduction |
| 221252 | pMSCV-FKBP-TRIF(trunc 1-123) | Expression of TRIF truncation 6 (124-222) in mammalian cells by retroviral transduction |
| 221253 | pMSCV-FKBP-TRIF(trunc 1-143) | Expression of TRIF truncation 7 (144-222) in mammalian cells by retroviral transduction |
| 221254 | pMSCV-FKBP-TRIF(trunc 1-163) | Expression of TRIF truncation 8 (164-222) in mammalian cells by retroviral transduction |
| 221255 | pMSCV-FKBP-TRIF(trunc 1-183) | Expression of TRIF truncation 9 (184-222) in mammalian cells by retroviral transduction |
| 221256 | pMSCV-FKBP-MAVS(pLxIS) | Expression of MAVS pLxIS (393-441) in mammalian cells by retroviral transduction |
| 221258 | pMSCV-FKBP-MAVS(pLxIS) E433A | Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction |
| 221259 | pMSCV-FKBP-MAVS(trunc Cterm) | Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction |
| 221260 | pMSCV-FKBP-IRF3 | Expression of IRF3 in mammalian cells by retroviral transduction |
| 221261 | pMSCV-FKBP-TASL(pLxIS) | Expression of TASL pLxIS (266-298) in mammalian cells by retroviral transduction |
| 221262 | pMSCV-FKBP-TASL(Cterm) | Expression of TASL Cterm (102-298) in mammalian cells by retroviral transduction |
| 221263 | pMSCV-FKBP-TASL(FL) | Expression of TASL in mammalian cells by retroviral transduction |
| 221264 | pMSCV-FKBP-TASL(pLxIS-PLPLR) | Expression of TASL pLxIS-STING PLPLR in mammalian cells by retroviral transduction |
| 221265 | pMSCV-TASL | Expression of TASL in mammalian cells by retroviral transduction |
| 221266 | pMSCV-TASL-PLPLR | Expression of TASL-STING PLPLR in mammalian cells by retroviral transduction |
| 221267 | pMSCV-TASL-NEMO | Expression of TASL-NEMO IKK binding site in mammalian cells by retroviral transduction |
| 221268 | pMSCV-FKBP-IRSp53(pLxIS) | Expression of IRSp53 pLxIS (247-375) in mammalian cells by retroviral transduction |
| 221269 | pMSCV-FKBP-IRSp53(pLxIS) S269A | Expression of IRSp53 pLxIS mutant in mammalian cells by retroviral transduction |
| 221270 | pMSCV-FKBP-GMIP(pLxIS) | Expression of GMIP pLxIS (1-84) in mammalian cells by retroviral transduction |
| 221271 | pMSCV-FKBP-GMIP(pLxIS) S31A | Expression of GMIP pLxIS mutant in mammalian cells by retroviral transduction |
| 221272 | pMSCV-FKBP-IRSp53(trunc N1) | Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction |
| 221273 | pMSCV-FKBP-IRSp53(trunc C1) | Expression of IRSp53 C-terminal truncation 1 (247-355) in mammalian cells by retroviral transduction |
| 221274 | pMSCV-FKBP-IRSp53(trunc C2) | Expression of IRSp53 C-terminal truncation 2 (247-335) in mammalian cells by retroviral transduction |
| 221276 | pMSCV-FKBP-IRSp53(trunc C4) | Expression of IRSp53 C-terminal truncation 4 (247-295) in mammalian cells by retroviral transduction |
| 221277 | pMSCV-FKBP-IRSp53(trunc C5) | Expression of IRSp53 C-terminal truncation 5 (247-275) in mammalian cells by retroviral transduction |
| 221278 | pMSCV-FKBP-GMIP(trunc N1) | Expression of GMIP N-terminal truncation 1 (16-84) in mammalian cells by retroviral transduction |
| 221279 | pMSCV-FKBP-GMIP(trunc C1) | Expression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transduction |
| 221280 | pMSCV-FKBP-GMIP(trunc C2) | Expression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transduction |
| 221281 | pMSCV-FKBP-IRSp53 with STING pLxIS | Expression of IRSp53 with STING pLxIS (NLV265RLL) in mammalian cells by retroviral transduction |
| 221282 | pMSCV-FKBP-STING with IRSp53 pLxIS | Expression of STING with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction |
| 221283 | pMSCV-FKBP-STING L373A with IRSp53 pLxIS | Expression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction |
| 221284 | pMSCV-FKBP-RFP(pLxIS-PLPLR) | Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction |
| 221285 | pMSCV-FKBP-Myoglobin(pLxIS-PLPLR) | Expression of Myoglobin (pLxIS-PLPLR) in mammalian cells by retroviral transduction |
| 221286 | pMSCV-FKBP-p27(pLxIS-PLPLR) | Expression of p27 (pLxIS-PLPLR) in mammalian cells by retroviral transduction |
| 221287 | pMSCV-FKBP-H3(pLxIS-PLPLR) | Expression of H3 (pLxIS-PLPLR) in mammalian cells by retroviral transduction |
| 221288 | pMSCV-FKBP-CNOT6 | Expression of CNOT6 (pLxIS) in mammalian cells by retroviral transduction |
| 221289 | pMSCV-FKBP-DAGLA | Expression of DAGLA (pLxIS) in mammalian cells by retroviral transduction |
| 221290 | pMSCV-FKBP-DZIP1L | Expression of DZIP1L (pLxIS) in mammalian cells by retroviral transduction |
| 221291 | pMSCV-FKBP-GARNL3 | Expression of GARNL3 (pLxIS) in mammalian cells by retroviral transduction |
| 221292 | pMSCV-FKBP-JCAD | Expression of JCAD (pLxIS) in mammalian cells by retroviral transduction |
| 221293 | pMSCV-FKBP-KIF5C | Expression of KIF5C (pLxIS) in mammalian cells by retroviral transduction |
| 221295 | pMSCV-FKBP-MAP2K4 | Expression of MAP2K4 (pLxIS) in mammalian cells by retroviral transduction |
| 221296 | pMSCV-FKBP-NMD3 | Expression of NMD3 (pLxIS) in mammalian cells by retroviral transduction |
| 221297 | pMSCV-FKBP-NSD3 | Expression of NSD3 (pLxIS) in mammalian cells by retroviral transduction |
| 221298 | pMSCV-FKBP-TMPRSS3 | Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction |
| 221299 | pMSCV-FKBP-ZBTB44 | Expression of ZBTB44 (pLxIS) in mammalian cells by retroviral transduction |
| 221300 | pMSCV-FKBP-ZC3H12B | Expression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction |
| 221301 | pMSCV-FKBP-ARHGAP45 | Expression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transduction |
| 221303 | pMSCV-FKBP-ASAP2 | Expression of ASAP2 (pLxIS) in mammalian cells by retroviral transduction |
| 221304 | pMSCV-FKBP-ASAP3(Cterm) | Expression of ASAP3 (Cterm pLxIS) in mammalian cells by retroviral transduction |
| 221307 | pMSCV-FKBP-FES | Expression of FES (pLxIS) in mammalian cells by retroviral transduction |
| 221309 | pMSCV-FKBP-NOSTRIN | Expression of NOSTRIN (pLxIS) in mammalian cells by retroviral transduction |
| 221310 | pMSCV-FKBP-OPHN1 | Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction |
| 221311 | pMSCV-FKBP-SYNDAPIN2 | Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction |
| 221312 | pMSCV-FKBP-TUBA | Expression of TUBA (pLxIS) in mammalian cells by retroviral transduction |
| 221313 | pMSCV-MAVS | Expression of MAVS in mammalian cells by retroviral transduction |
| 221314 | pMSCV-MAVS(pLxIS trunc) | Expression of MAVS truncated pLxIS domain in mammalian cells by retroviral transduction |
| 221315 | pMSCV-MAVS(IRSp53 pLxIS) | Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction |
| 221316 | pMSCV-MAVS(GMIP pLxIS) | Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction |
| 221317 | pRetroX-TRE3G-IRSp53 | Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction |
| 221318 | pRetroX-TRE3G-GMIP | Dox-inducible expression of GMIP in mammalian cells by retroviral transduction |
| 221319 | pRetroX-TRE3G-IRSp53 S269D | Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction |
| 221320 | pRetroX-TRE3G-IRSp53 P278D P281D | Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction |
| 221321 | pRetroX-TRE3G-IRSp53 P428L | Dox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction |
| 221322 | pRetroX-TRE3G-GMIP S31D | Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction |
| 221323 | pRetroX-TRE3G-GMIP P363D P364D P366D | Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction |
| 221324 | pRetroX-TRE3G-GMIP P564D | Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction |
| 221325 | pRetroX-TRE3G-Gag | Dox-inducible expression of Gag in mammalian cells by retroviral transduction |
| 221326 | pRetroX-TRE3G-Tir | Dox-inducible expression of Tir in mammalian cells by retroviral transduction |
| 221327 | pRetroX-TRE3G-SopE | Dox-inducible expression of SopE in mammalian cells by retroviral transduction |
| 221328 | pRetroX-TRE3G-YopE | Dox-inducible expression of YopE in mammalian cells by retroviral transduction |
| 221329 | pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V) | Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction |
| 221330 | pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V) | Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction |
| 221331 | pFastBac-GST-TBK1 | TBK1 protein overexpression in Sf9 insect cells |
| 221332 | pET28a-FKBP-empty | Protein overexpression in bacteria |
| 221333 | pET28a-FKBP-STING(pLxIS) | Overexpression of STING CTT in bacteria |
| 221334 | pET28a-FKBP-TRIF(Nterm) | Overexpression of TRIF Nterm domain in bacteria |
| 221335 | pET28a-FKBP-MAVS(pLxIS) | Overexpression of MAVS pLxIS domain in bacteria |
| 221336 | pET28a-FKBP-IRSp53(pLxIS) | Overexpression of IRSp53 pLxIS domain in bacteria |
| 221337 | pET28a-FKBP-GMIP(pLxIS) | Overexpression of GMIP pLxIS domain in bacteria |