Helicase-assisted continuous editing for programmable mutagenesis of endogenous genomes
Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F
bioRxiv 2024.02.01.577593 Article
Chen XD, Chen Z, Wythes G, Zhang Y, Orr BC, Sun G, Thao K, Vallurupalli M, Sun J, Borji M, Tkacik E, Chen H, Bernstein BE, Chen F
bioRxiv 2024.02.01.577593 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221218 | pCMV-AID-BLM-UGI | Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase. |
| 221219 | pCMV-AID-Ns3h-UGI | Express HACE Editor as a fusion of AID cytidine deaminase and Ns3h helicase. |
| 221220 | pCMV-AID-PcrA-UGI | Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase. |
| 221222 | pCMV-AID-BLM-UGI-T2A-mTagBFP2 | Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter |
| 221227 | pCMV-TadA8e-Ns3h | Express HACE Editor as a fusion of TadA-8e adenosine deaminase and Ns3h helicase. |
| 221228 | pCMV-TadA8e-PcrA | Express HACE Editor as a fusion of TadA-8e adenosine deaminase and PcrA helicase. |
| 221231 | pCMV-SpCas9(H840A)-T2A-EGFP | Express nCas9 nickase (H840A) with GFP reporter |
| 221232 | U6-BbsI-GGsite-mCherry-Puro | Cloning vector for sgRNA with mCherry and Puro |
| 221233 | U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP | Express sgRNA targeting HEK3 loci with nCas9(D10A) |
| 221235 | tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA | Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase. |
| 221236 | pCMV-tadDE-PcrA M6-UGI | Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase. |