Nucleosome recognition and DNA distortion by the Chd1 remodeler in a nucleotide-free state.
Nodelman IM, Das S, Faustino AM, Fried SD, Bowman GD, Armache JP
Nat Struct Mol Biol. 2022 Feb;29(2):121-129. doi: 10.1038/s41594-021-00719-x. Epub 2022 Feb 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221196 | pGEM-601[insert(+1) TA-rich] | 601 nucleosome positioning sequence with an additional base pair inserted 22 nt from the dyad on the TA-rich side of the 601 |
| 221197 | pDEST17-ScChd1[R126A/R130A] | ScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutations |
| 221198 | pDEST17-ScChd1[Y137A] | ScChd1-aa118-1274 chromatin remodeler with Y137A mutation |
| 221199 | pDEST17-ScChd1[∆ChEx] | ScChd1-aa142-1274 chromatin remodeler with ChEx domain deleted |
| 221200 | pET3a-XlH3[ChEx] | Xenopus laevis histone H3 with Nterminal tail swapped for ChEx domain of ScChd1. |
| 221201 | pET3a-Xl-H3∆Nterm[R42] | Xenopus laevis histone H3 with N-terminal tail deleted. |
| 221207 | pDEST17-ScChd1(118-1274) | ScChd1-aa118-1274 chromatin remodeler |