A genetically encoded biosensor to monitor dynamic changes of c-di-GMP with high temporal resolution.
Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, Reinders A, Klotz A, Maier T, Jenal U
Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0. PubMed Article
Kaczmarczyk A, van Vliet S, Jakob RP, Teixeira RD, Scheidat I, Reinders A, Klotz A, Maier T, Jenal U
Nat Commun. 2024 May 9;15(1):3920. doi: 10.1038/s41467-024-48295-0. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221142 | p2H12 | cdGreen expressed from an aTc-inducible promoter (Internal Lab ID: UJ11204) |
| 221143 | p2H12Matry | cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208) |
| 221145 | pET28-2H12.D11 | His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357) |
| 221150 | pBBR15.2-2H12.D11opt-scarRef | cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241) |
| 221151 | pConRef-2H12.D11 | cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236) |
| 221181 | p2H12Matry-blind | cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209) |
| 221182 | pConRef-2H12 | cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213) |
| 221184 | pQFmcs-2H12.D11-scarREF | cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240) |
| 221185 | pBBR15.2-2H12.D11opt | cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239) |
| 221186 | pET28-2H12 | His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308) |
| 221187 | pET28-2H12Matry | His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309) |
| 221188 | p2H12ref | cdGreen expressed in operon with mScarlet-I from an aTc-inducible promoter (Internal Lab ID: UJ11206) |