CRISPR-Cas9-based approaches for genetic analysis and epistatic interaction studies in Coxiella burnetii.
Steiner S, Roy CR
mSphere. 2024 Nov 19:e0052324. doi: 10.1128/msphere.00523-24.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221135 | pHelper-CRISPRi-sgRNA | Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning. |
| 221136 | pCB-CRISPRi | Coxiella burnetii CRISPRi plasmid without sgRNA construct. Expresses 3xF-dCas9. |
| 221137 | pHelper-CBE-sgRNA | Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning. |
| 221138 | pCB-CBEv1 | Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3. |
| 221139 | pCB-CBEv2 | Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A). |