Optimization of the fluorogen-activating protein tag for quantitative protein trafficking and colocalization studies in S. cerevisiae.
Oppenheimer KG, Hager NA, McAtee CK, Filiztekin E, Shang C, Warnick JA, Bruchez MP, Brodsky JL, Prosser DC, Kwiatkowski AV, O'Donnell AF
Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi: 10.1091/mbc.E24-04-0174. Epub 2024 May 29.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221086 | pRS415-TEF1pr-NFAPorig | FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag) |
| 221087 | pRS415-TEF1pr-CFAPorig | FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag) |
| 221088 | pRS415-TEF1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221089 | pRS415-TEF1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221090 | pRS415-TEF1pr-MFA-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER |
| 221091 | pRS413-TEF1pr-NFAPorig | FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag) |
| 221092 | pRS413-TEF1pr-CFAPorig | FAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag) |
| 221093 | pRS413-TEF1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221094 | pRS413-TEF1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221095 | pRS413-TEF1pr-MFA-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER |
| 221096 | pRS415-ADH1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221097 | pRS415-ADH1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221098 | pRS413-ADH1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221099 | pRS413-ADH1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)+E2:E15 |
| 221100 | pRS415-MET25pr | Methionine-repressible gene expression |
| 221101 | pRS415-MET25pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221102 | pRS415-MET25pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221103 | pRS413-MET25pr | Methionine-repressible gene expression |
| 221104 | pRS413-MET25pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221105 | pRS413-MET25pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221106 | pRS415-CUP1pr | Copper-inducible gene expression |
| 221107 | pRS415-CUP1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221108 | pRS415-CUP1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221109 | pRS413-CUP1pr | Copper-inducible gene expression |
| 221110 | pRS413-CUP1pr-NFAPoptim | FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221111 | pRS413-CUP1pr-CFAPoptim | FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) |
| 221112 | pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3 | FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER |
| 221113 | pRS415-TEF1pr-STE3-GFP | GFP-tagged STE3 (C-terminally tagged, optimized for yeast expression) |
| 221114 | pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3 | FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER |
| 221115 | pRS415-TEF1pr-SEC61-FAPoptim | FAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag |
| 221116 | pRS415-TEF1pr-SEC63-FAPoptim | FAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tag |
| 221117 | pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS | FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER |
| 221118 | pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-deltaPEST | FAP tagged STE3-PEST mutant (delta413-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER |
| 221119 | pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-K424R | FAP tagged STE3-K424R mutant (N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER |
| 221120 | pRS413-TEF1pr-ANP1-FAPoptim | FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression) |
| 221121 | pRS413-TEF1pr-ERG6-FAPoptim | FAP-tagged marker for lipid droplets (FAP optimized for yeast expression) |
| 221122 | pRS413-TEF1pr-PMA1-FAPoptim | FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression) |
| 221123 | pRS413-TEF1pr-RPA34-FAPoptim | FAP-tagged marker for the nucleus (FAP optimized for yeast expression) |
| 221124 | pRS413-TEF1pr-SEC7-FAPoptim | FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression) |
| 221125 | pRS413-TEF1pr-VPH1-FAPoptim | FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression) |
| 221126 | pRS413-TEF1pr-SEC61-FAPoptim | FAP-tagged marker for the ER (FAP optimized for yeast expression) |
| 221127 | pDP0437 | Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX |
| 221128 | pFA6-CFAPoptim-KAN | Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX |
| 221129 | pFA6-CFAPoptim-NAT | Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX |