CRISPR-dCas13d-based deep screening of proximal and distal splicing-regulatory elements.
Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu Q, Zhang C
Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214430 | pDualIN-SMN2 | Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7 |
| 214431 | pDualEX-SMN2 | Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7 |
| 215720 | dCas13d-BFP | Catalytically inactive dCasRx with 2A-BFP |
| 215721 | pDual-IN-SMN1 | dual-color reporter express eGFP constitutively ; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7; expresses SMN1 |
| 221085 | pTomatoSlicer | Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter. |