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Massively parallel evaluation and computational prediction of the activities and specificities of 17 small Cas9s.

Seo SY, Min S, Lee S, Seo JH, Park J, Kim HK, Song M, Baek D, Cho SR, Kim HH
Nat Methods. 2023 Jul;20(7):999-1009. doi: 10.1038/s41592-023-01875-2. Epub 2023 May 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220964pLenti6.3-sRGN3.1-BlastRExpresses human codon-optimized sRGN3.1
220965pLenti6.3-SlugCas9-BlastRExpresses human codon-optimized SlugCas9
220966pLenti6.3-SaCas9-BlastRExpresses human codon-optimized SaCas9
220967pLenti6.3-SauriCas9-BlastRExpresses human codon-optimized SauriCas9
220968pLenti6.3-Sa-SlugCas9-BlastRExpresses human codon-optimized Sa-SlugCas9
220971pLenti6.3-efSaCas9-BlastR Expresses human codon-optimized efSaCas9
220972pLenti6.3-SauriCas9-KKH-BlastRExpresses human codon-optimized SauriCas9-KKH
220973pLenti6.3-SlugCas9-HF-BlastRExpresses human codon-optimized SlugCas9-HF
220976pLenti6.3-St1Cas9-BlastRExpresses human codon-optimized St1Cas9
220978pLenti6.3-enCjCas9-BlastRExpresses human codon-optimized enCjCas9
220979pLenti6.3-CjCas9-BlastRExpresses human codon-optimized CjCas9
220980pLenti6.3-Nm2Cas9-BlastRExpresses human codon-optimized Nm2Cas9

Antibodies from Article