Analyzing mRNA-protein complexes using a yeast three-hybrid system.
Bernstein DS, Buter N, Stumpf C, Wickens M
Methods. 2002 Feb;26(2):123-41. doi: 10.1016/S1046-2023(02)00015-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220627 | pMS2-1 | Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites. |
| 220628 | pMS2-2 | Plasmid allows you to place the RNA sequence of interest next to two MS2 coat protein recognition sites. |
| 220629 | pIII/MS2-1 | Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites. |
| 220630 | pIII/MS2-2 | For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter. |
| 220631 | pIIIA/MS2-1 | For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter. |
| 220632 | pIIIA/MS2-2 | For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter. |
| 220738 | p3HR2 | Component of yeast three hybrid system |
| 220751 | pAD-IRP | Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain. |