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The NK cell receptor NKp46 recognizes ecto-calreticulin on ER-stressed cells.

Sen Santara S, Lee DJ, Crespo A, Hu JJ, Walker C, Ma X, Zhang Y, Chowdhury S, Meza-Sosa KF, Lewandrowski M, Zhang H, Rowe M, McClelland A, Wu H, Junqueira C, Lieberman J
Nature. 2023 Apr;616(7956):348-356. doi: 10.1038/s41586-023-05912-0. Epub 2023 Apr 5. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
221853pCMV-CRT-Fc FLExpress human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.
221854pCMV-CRT-Fc NExpress human Calreticulin truncated N domain with C-terminal human IgG1Fc tag in mammalian cell.
221855pCMV-CRT-Fc PExpress human Calreticulin truncated P domain with C-terminal human IgG1Fc tag in mammalian cell.
221856pCMV-CRT-Fc CExpress human Calreticulin truncated C domain with C-terminal human IgG1Fc tag in mammalian cell.
221857pCMV-NKp46-FcExpress human NKp46 (full-length) with C-terminal human IgG1Fc tag in mammalian cell.
223711pRetroX-mCRT-GPI-IRES-DsRedMammalian expression of mouse CRT-GPI

Antibodies from Article