In vitro transcription-based biosensing of glycolate for prototyping of a complex enzyme cascade
Barthel S, Brenker L, Diehl C, Bohra N, Giaveri S, Paczia N, Erb TJ
Synthetic Biology, 2024;, ysae013
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 221189 | pTE5400_pMBP_CDS dropout vector | Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest |
| 221191 | pTE5417_p3WJdB_promoter dropout vector | Promoter probe vector with dropout sequence for promoter sequences with 3WJdB reporter |
| 221192 | pTE5418_pMBP-GlcR | Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli. |
| 221193 | pTE5419_p3WJdB-glcO36 | GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator |
| 221194 | pTE5423_p3WJdB-glcO60 | DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system |