Alex Borodavka Lab: Rotavirus Nonstructural Protein (NSP) plasmids
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220802 | pT7-NSP5ASA11 | Recoded gene segment 11 mutant A strain SA11 used for genome packaging studies |
| 220803 | pT7-NSP5BSA11 | Recoded gene segment 11 mutant B strain SA11 used for genome packaging studies |
| 220804 | pT7-NSP5CSA11 | Recoded gene segment 11 mutant C strain SA11 used for genome packaging studies |
| 220805 | pT7-NSP5DSA11 | Recoded gene segment 11 mutant D strain SA11 used for genome packaging studies |
| 220806 | pT7-NSP5ESA11 | Recoded gene segment 11 mutant E strain SA11 used for genome packaging studies |
| 220807 | pT7-NSP5XSA11 | Recoded gene segment 11 mutant X strain SA11 used for genome packaging studies |
| 220808 | pT7-NSP3SA11-F2A-EGFP | Express separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996) |
| 220809 | pT7-NSP3SA11-P2A-NSP5(wt)-SNAP | Express separately the Rotavirus SA11 NSP3 protein and the Rotavirus SA11 NSP5 fused with SNAP-tag |
| 220810 | pT7-NSP3SA11-P2A-C1C-EGFP | Express separately the Rotavirus SA11 NSP3 protein and C1C-EGFP |
| 220811 | pT7-NSP1delta103CSA11 | Express the Rotavirus SA11 delta103C NSP1 protein |
| 220812 | pT7-NSP1delta42CSA11 | Express the Rotavirus SA11 delta42C NSP1 protein |
| 220813 | pT7-NSP1delta103CSA11-GFP | Express the Rotavirus SA11 delta103C NSP1 protein fused with the sfGFP (Pedelacq et al., 2006) |
| 220814 | pT7-NSP1delta103CSA11-P2A-GFP | Express separately the Rotavirus SA11 delta103C NSP1 protein and the sfGFP (Pedelacq et al., 2006) |
| 220816 | pT7-NSP1RF(ioSA11) | Express the Rotavirus RF NSP1 recoded protein |
| 220817 | pT7-NSP1DS1 | Express the Rotavirus DS-1 NSP1 protein |
| 220818 | pT7-NSP1DS1(ioSA11) | Express the Rotavirus DS-1 NSP1 recoded protein |
| 220819 | pT7-NSP1RRV | Express the Rotavirus RRV NSP1 protein |
| 220820 | pT7-NSP4SA11-hPEST | Express the Rotavirus SA11 NSP4 fused with the PEST degradation sequence from mouse ornithine decarboxylase |
| 220821 | pT7-35aaNSP1SA11-Green Lantern(wt) | Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini |
| 220822 | pT7-35aaNSP1SA11-Green Lantern(io) | Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the recoded Green Lantern protein carrying an ER-localization signal at the N- and C-termini |
| 220823 | pT7-35aaNSP1SA11-NSP5(wt) | Express the protein consisting of 35 amino acid of Rotavirus SA11 NSP1 protein and the full-length SA11 NSP5 protein |
| 220824 | pT7-35aaNSP1SA11-NSP5(io) | Express the protein consisting of 35 amino acid of Rotavirus SA11 NSP1 protein and the full-length recoded NSP5 protein |
| 220825 | pT7-35aaNSP1SA11 | Express the short Rotavirus SA11 NSP1 protein (35 amino acids) |
| 220826 | pT7-35aaNSP1noMSA11-NSP5(wt) | Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length SA11 NSP5 protein |
| 220827 | pT7-35aaNSP1noMSA11-NSP5(io) | Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length recoded NSP5 protein |
| 220828 | pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP | Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP |
| 220829 | pT7-NSP5SClow | Express the Rotavirus NSP5 protein with a potentially lower propensity to form viroplasms |