Macrolide Biosensor Optimization through Cellular Substrate Sequestration.
Miller CA, Ho JM, Parks SE, Bennett MR
ACS Synth Biol. 2021 Feb 19;10(2):258-264. doi: 10.1021/acssynbio.0c00572. Epub 2021 Feb 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220830 | pCM54 | EryA sensor with increased sensitivity due to phosphorylation of EryA by mphA, which traps EryA in cell |
| 220831 | pCM98 | EryA sensor with further increased sensitivity by mutation of mphR (T17R), in addition to mphA phosphorylation |
| 220832 | pCM120 | EryA sensor with increased sensitivity by mphA and high level resistance by ermC |
| 220833 | pCM121 | EryA sensor with increased sensitivity (mphA and mphR (T17R) mutation) and high level resistance (ermC) |