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Destabilized near-infrared fluorescent nanobodies enable background-free targeting of GFP-based biosensors for imaging and manipulation

Barykina N et al.
Nat Commun 15, 7788 (2024) (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220739pNIR-FbLAG16NIR-FbLAG16 expression in mammalian cells.
220740pNIR-FbLAG30NIR-FbLAG30 expression in mammalian cells.
220742pNIR-Fb(718)NIR-Fb(718) expression in mammalian cells.
220743pGAL4-sfGFP(1-157)GAL4-sfGFP(1-157) construct expression in mammlian cells.
220744pGAL4-sfGFP(158-238)GAL4-sfGFP(158-238) construct expression in mammlian cells.
220745pNLS-VP16-NIR-Fb(718)NLS-VP16-NIR-Fb(718) consrtuct expression in mammalian cells.
220746pGAL4-NIR-FbLAG30GAL4-NIR-FbLAG30 construct expression in mammlian cells.
220747pGP-mTagBFP2mTagBFP2 expression in mammalian cells. Reporter plasmid.
220748pET-NIR-FbLAG30NIR-FbLAG30 expression in bacteria.
220749pAAV-CAG-NIR-FbLAG16NIR-FbLAG16 expression in mammalian cells. pAAV vector.
220750pAAV-CAG-NIR-FbLAG30NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Antibodies from Article