Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase.
Preston MA, Porter DF, Chen F, Buter N, Lapointe CP, Keles S, Kimble J, Wickens M
Nat Methods. 2019 May;16(5):437-445. doi: 10.1038/s41592-019-0370-6. Epub 2019 Apr 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220622 | Leu2 2u | empty backbone for shuttle vector construction |
| 220623 | MAP72_MS2 | Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae. |
| 220624 | MAP80A_MS2 | tRNA reporter vector |
| 220625 | pTEF1_MS2 | Source for TEF1 MS2 coat fusion protein fusion |