Light induced expression of gRNA allows for optogenetic gene editing of T lymphocytes in vivo.
Pulgarin DV, Pelo N, Ferrandiz L, Trselic T, Nyberg WA, Bowlin G, Espinosa A
bioRxiv [Preprint]. 2023 Nov 10:2023.11.09.566272. doi: 10.1101/2023.11.09.566272.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220497 | pMSCV-BLU-VIPR | Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR). |
| 220498 | pBLU-VIPR | Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR). |