Identification of the main barriers to Ku accumulation in chromatin.
Bossaert M, Moreno A, Peixoto A, Pillaire MJ, Chanut P, Frit P, Calsou P, Loparo JJ, Britton S
bioRxiv [Preprint]. 2024 Jan 4:2024.01.03.574002. doi: 10.1101/2024.01.03.574002.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220493 | pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs | To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1. |
| 220494 | pCAG-eSpCas9-2A-GFP-sgRNA-Lig4 | To generate LIG4 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against LIG4 exon 3. |
| 220495 | pUC18-GFP | To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity. |
| 220496 | pUC18-LucFF | To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity. |