Targeting T cell plasticity in kidney and gut inflammation by pooled single-cell CRISPR screening.
Enk LUB, Hellmig M, Riecken K, Kilian C, Datlinger P, Jauch-Speer SL, Neben T, Sultana Z, Sivayoganathan V, Borchers A, Paust HJ, Zhao Y, Asada N, Liu S, Agalioti T, Pelczar P, Wiech T, Bock C, Huber TB, Huber S, Bonn S, Gagliani N, Fehse B, Panzer U, Krebs CF
Sci Immunol. 2024 Jun 14;9(96):eadd6774. doi: 10.1126/sciimmunol.add6774. Epub 2024 Jun 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220472 | CROPseq-MPSV-eBFP2 | Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection |