HDI-STARR-seq: Condition-specific enhancer discovery in mouse liver in vivo.
Chang TY, Waxman DJ
BMC Genomics. 2024 Dec 24;25(1):1240. doi: 10.1186/s12864-024-11162-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220282 | pGYL1_pPromALB_FireflyLuc | Firefly luciferase with minimal Albumin promoter without enhancer; enhancer of interest can be cloned into MCS |
| 220283 | pGYL17_pPromALB_enhALB_FireflyLuc | Firefly luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pPromALB_FireflyLuc |
| 220284 | pGYL18_pPromALB_enhALB_RenillaLuc | Renilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega) |
| 220285 | pTYC1_pPromALB_enh5898_FireflyLuc | Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc |
| 220286 | pTYC2_pPromALB_enh29774_FireflyLuc | Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc |
| 220287 | pTYC3_pPromALB_enh50363_FireflyLuc | Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc |
| 220288 | pTYC4_pPromALB_enh50358_FireflyLuc | Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc |
| 220289 | pTYC10_pPromALB_STARRseq | STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver. |