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Inseparable RNA binding and chromatin modification activities of a nucleosome-interacting surface in EZH2.

Gail EH, Healy E, Flanigan SF, Jones N, Ng XH, Uckelmann M, Levina V, Zhang Q, Davidovich C
Nat Genet. 2024 May 14. doi: 10.1038/s41588-024-01740-8. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220239pFB1.HMBP.PrS.EZH2-mt1Expression of an EZH2 mutant in insect cells.
220240pFB1.HMBP.PrS.EZH2-mt2Expression of an EZH2 mutant in insect cells.
220241pFB1.HMBP.PrS.dEZH2Expression of an EZH2 catalytic site mutant in insect cells.
220243pHIV-EGFP.Flag-EZH2Expresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.
220244pHIV-EGFP.Flag-EZH2-mt1Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.
220245pHIV-EGFP.Flag-EZH2-mt2Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.
220246pHIV-EGFP.Flag-EZH2-mt2*Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.
220318pXPR_003.gEZH2Expresses guide RNA for CRISPR/Cas9 knockout of EZH2 for knockout/rescue experiments in mammalian cells.

Antibodies from Article