TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts.
Fielden J, Wiseman K, Torrecilla I, Li S, Hume S, Chiang SC, Ruggiano A, Narayan Singh A, Freire R, Hassanieh S, Domingo E, Vendrell I, Fischer R, Kessler BM, Maughan TS, El-Khamisy SF, Ramadan K
Nat Commun. 2020 Mar 9;11(1):1274. doi: 10.1038/s41467-020-15000-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220204 | pCDNA3-TEX264wt-cFlag | Mammalian expression of wild type TEX264, C-terminal Flag |
| 220205 | pCDNA3-TEX264-SIM1_mut-cFlag | Mammalian expression of TEX264 with SIM1 motif mutated, C-terminal Flag |
| 220206 | pCDNA3-TEX264-SIM2_mut-cFlag | Mammalian expression of TEX264 with SIM2 motif mutated, C-terminal Flag |
| 220207 | pCDNA3-TEX264-E194A-cFlag | Mammalian expression of TEX264 with E194A mutation, C-terminal Flag |
| 220208 | pCDNA3-TEX264-SHP1mut-cFlag | Mammalian expression of TEX264 with SHP domain mutated, C-terminal Flag |
| 220209 | pCDNA5 TEX264wt-cSSH | Mammalian expression of wild type TEX264, C-terminal twin-Strep_HA tag |
| 220210 | pCDNA5-TEX264-deltaLRR-cSSH | Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag |
| 220211 | pCDNA5 TEX264wt-cGFP | Mammalian expression of wild type TEX264 fused at C-terminus to GFP |
| 220212 | pCDNA5-TEX264-SIM1_mut-cGFP | Mammalian expression of TEX264 with SIM1 motif mutated, fused at C-terminus to GFP |
| 220213 | pCDNA5-TEX264-SIM2_mut-cGFP | Mammalian expression of TEX264 with SIM2 motif mutated, fused at C-terminus to GFP |
| 220214 | pET21a-TEX264_wt-His6 | Bacterial expression of wild type TEX264, C-terminal His6 tag |
| 220215 | pET21a-TEX264-deltaLRR-His6 | Bacterial expression of TEX264, 2-25 residues (LRR) truncated, C-terminal His6 tag |