Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly.
Roghair Stroud MN, Vang DX, Halverson LJ
ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220184 | pSpy | sgRNA expression vector - promoterless backbone |
| 220185 | pSpyA | sgRNA expression vector - pEM7 promoter |
| 220186 | pSpyB | sgRNA expression vector - pBG35 promoter |
| 220187 | pSpyC | sgRNA expression vector - pBG42 promoter |
| 220188 | pSpyD | sgRNA expression vector - pBG28 promoter |
| 220189 | pSpyA-GFP | sgRNA expression vector - pEM7 promoter with GFP in sgRNA site |
| 220190 | pSpyB-GFP | sgRNA expression vector - pBG35 promoter with GFP in sgRNA site |
| 220191 | pSpyC-GFP | sgRNA expression vector - pBG42 promoter with GFP in sgRNA site |
| 220192 | pSpyD-GFP | sgRNA expression vector - pBG28 promoter with GFP in sgRNA site |
| 220193 | pMRS-dCas9 | mini-Tn7 dCas9 expression vector |
| 220194 | pMRS-LacI-dCas9 | mini-Tn7 dCas9 expression vector - LacI/pLac promoter |
| 220195 | pMRS-AraC-dCas9 | mini-Tn7 dCas9 expression vector - AraC/pBAD promoter |
| 220196 | pMRS-XylS-dCas9 | mini-Tn7 dCas9 expression vector - XylS/pM promoter |
| 220197 | pMRS-GFP | mini-Tn7 GFP expression vector |
| 220198 | pMRS-LacI-GFP | mini-Tn7 GFP expression vector - LacI/pLac promoter |
| 220199 | pMRS-AraC-GFP | mini-Tn7 GFP expression vector - AraC/pBAD promoter |
| 220200 | pMRS-XylS-GFP | mini-Tn7 GFP expression vector - XylS/pM promoter |
| 220201 | pMRS-BG35-GFP | mini-Tn7 GFP expression vector - pBG35 promoter |
| 220202 | pMRS-BG42-GFP | mini-Tn7 GFP expression vector - pBG42 promoter |
| 220203 | pMRS-empty | mini-Tn7 cloning vector - empty backbone |