Optimized CRISPR Interference System for Investigating Pseudomonas alloputida Genes Involved in Rhizosphere Microbiome Assembly.
Roghair Stroud MN, Vang DX, Halverson LJ
ACS Synth Biol. 2024 Aug 20. doi: 10.1021/acssynbio.4c00312.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
220184 | pSpy | sgRNA expression vector - promoterless backbone |
220185 | pSpyA | sgRNA expression vector - pEM7 promoter |
220186 | pSpyB | sgRNA expression vector - pBG35 promoter |
220187 | pSpyC | sgRNA expression vector - pBG42 promoter |
220188 | pSpyD | sgRNA expression vector - pBG28 promoter |
220189 | pSpyA-GFP | sgRNA expression vector - pEM7 promoter with GFP in sgRNA site |
220190 | pSpyB-GFP | sgRNA expression vector - pBG35 promoter with GFP in sgRNA site |
220191 | pSpyC-GFP | sgRNA expression vector - pBG42 promoter with GFP in sgRNA site |
220192 | pSpyD-GFP | sgRNA expression vector - pBG28 promoter with GFP in sgRNA site |
220193 | pMRS-dCas9 | mini-Tn7 dCas9 expression vector |
220194 | pMRS-LacI-dCas9 | mini-Tn7 dCas9 expression vector - LacI/pLac promoter |
220197 | pMRS-GFP | mini-Tn7 GFP expression vector |
220198 | pMRS-LacI-GFP | mini-Tn7 GFP expression vector - LacI/pLac promoter |
220199 | pMRS-AraC-GFP | mini-Tn7 GFP expression vector - AraC/pBAD promoter |
220200 | pMRS-XylS-GFP | mini-Tn7 GFP expression vector - XylS/pM promoter |