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Triaging of alpha-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology.

Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, Schulte F, Voorhees RM, Guna A, Weissman JS
Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220166pGM8Expresses a reporter for an alpha-helical OMM protein
220167pGM9Expresses a reporter for an alpha-helical OMM protein
220168pGM117Expresses a reporter for an alpha-helical OMM protein
220169pGM63CRISRPi guide RNA against TOMM70A
220170pGM65CRISRPi guide RNA against NACA
220171pGM79CRISRPi guide RNA against MARCHF5
220172pGM84CRISRPi guide RNA against BTF3
220173pGM133CRISRPi guide RNA against TTC1
220174pGM209Expresses exogenous wild-type TTC1
220175pGM216Expresses exogenous TPR mutant TTC1 (R190A_R194A)
220176pGM258Expresses exogenous C-terminal mutant TTC1 (I271D)
220177pGM234Expresses exogenous wild-type NAC complex members (NACA and BTF3)
220178pGM238Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)
220179pGM265Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag
220180pGM266Expresses exogenous TPR mutant TTC1 (R190A_R194A) with an N-terminal sfGFP tag
220181pGM268Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag
220182pGM227Expresses exogenous wild-type TOMM70A
220183pGM295Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag
226738pAG207Expresses a reporter for an alpha-helical OMM protein
226742pAG27Expresses a reporter for a mitochondrial matrix protein
226744pAG155CRISPRi guide RNA against TOMM20
226745pAG156CRISPRi guide RNA against TOMM20
226746pAG157CRISPRi guide RNA against TOMM22
226751pAG66Expresses exogenous GFP

Antibodies from Article