Triaging of alpha-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology.

Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, Schulte F, Voorhees RM, Guna A, Weissman JS
Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
226751	pAG66	Expresses exogenous GFP
226746	pAG157	CRISPRi guide RNA against TOMM22
226745	pAG156	CRISPRi guide RNA against TOMM20
226744	pAG155	CRISPRi guide RNA against TOMM20
226742	pAG27	Expresses a reporter for a mitochondrial matrix protein
226738	pAG207	Expresses a reporter for an alpha-helical OMM protein
220183	pGM295	Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag
220182	pGM227	Expresses exogenous wild-type TOMM70A
220181	pGM268	Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag
220179	pGM265	Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag