Triaging of alpha-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology.
Muthukumar G, Stevens TA, Inglis AJ, Esantsi TK, Saunders RA, Schulte F, Voorhees RM, Guna A, Weissman JS
Mol Cell. 2024 Mar 21;84(6):1101-1119.e9. doi: 10.1016/j.molcel.2024.01.028. Epub 2024 Feb 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220166 | pGM8 | Expresses a reporter for an alpha-helical OMM protein |
| 220167 | pGM9 | Expresses a reporter for an alpha-helical OMM protein |
| 220171 | pGM79 | CRISRPi guide RNA against MARCHF5 |
| 220172 | pGM84 | CRISRPi guide RNA against BTF3 |
| 220173 | pGM133 | CRISRPi guide RNA against TTC1 |
| 220174 | pGM209 | Expresses exogenous wild-type TTC1 |
| 220175 | pGM216 | Expresses exogenous TPR mutant TTC1 (R190A_R194A) |
| 220176 | pGM258 | Expresses exogenous C-terminal mutant TTC1 (I271D) |
| 220177 | pGM234 | Expresses exogenous wild-type NAC complex members (NACA and BTF3) |
| 220178 | pGM238 | Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3) |
| 220179 | pGM265 | Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag |
| 220181 | pGM268 | Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag |
| 220182 | pGM227 | Expresses exogenous wild-type TOMM70A |
| 220183 | pGM295 | Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag |
| 226733 | pAG397 | Expresses a reporter for an alpha-helical OMM protein |
| 226734 | pAG426 | Expresses a reporter for an alpha-helical OMM protein |
| 226735 | pAG517 | Expresses a reporter for an alpha-helical OMM protein |
| 226736 | pAG43 | Expresses a reporter for an alpha-helical OMM protein |
| 226738 | pAG207 | Expresses a reporter for an alpha-helical OMM protein |
| 226742 | pAG27 | Expresses a reporter for a mitochondrial matrix protein |
| 226743 | pAG26 | Expresses a reporter for a mitochondrial intermembrane space protein |
| 226744 | pAG155 | CRISPRi guide RNA against TOMM20 |
| 226745 | pAG156 | CRISPRi guide RNA against TOMM20 |
| 226746 | pAG157 | CRISPRi guide RNA against TOMM22 |
| 226747 | pAG491 | CRISPRi guide RNA against MTCH2 (dual guide) |
| 226748 | pAG369 | CRISPRi guide RNA against EMC2 |
| 226749 | pAG392 | CRISPRi guide RNA against ATP13A1 |
| 226750 | pAG496 | Expresses exogenous MTCH2 |
| 226751 | pAG66 | Expresses exogenous GFP |