FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways.
Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
224377 | pX459_GNB1L_gRNA | Targets GNB1L for dTAG C terminal knock-in cell lines |
224378 | pUC19_GNB1L_dTAG | Generates GNB1L dTAG C terminal knock-in cell lines |
224379 | pX459_TELO2_gRNA | Targets TELO2 for dTAG N terminal knock-in cell lines |
224380 | pUC19_TELO2_dTAG | Generates TELO2 dTAG N terminal knock-in cell lines |
224381 | pLenti_CMV_Puro_SFB_GNB1L | Lenti plasmid for generating GNB1L expressing stable cell lines |
224385 | pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletion | Lenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines |
224387 | pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletion | Lenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines |
224388 | pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletion | Lenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines |