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FACS-based genome-wide CRISPR screens define key regulators of DNA damage signaling pathways.

Huang M, Yao F, Nie L, Wang C, Su D, Zhang H, Li S, Tang M, Feng X, Yu B, Chen Z, Wang S, Yin L, Mou L, Hart T, Chen J
Mol Cell. 2023 Aug 3;83(15):2810-2828.e6. doi: 10.1016/j.molcel.2023.07.004. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
224377pX459_GNB1L_gRNATargets GNB1L for dTAG C terminal knock-in cell lines
224378pUC19_GNB1L_dTAGGenerates GNB1L dTAG C terminal knock-in cell lines
224379pX459_TELO2_gRNATargets TELO2 for dTAG N terminal knock-in cell lines
224380pUC19_TELO2_dTAGGenerates TELO2 dTAG N terminal knock-in cell lines
224381pLenti_CMV_Puro_SFB_GNB1LLenti plasmid for generating GNB1L expressing stable cell lines
224385pLenti_CMV_Puro_SFB_GNB1L_WD 4 deletionLenti plasmid for generating GNB1L _WD 4 domain depleted expressing stable cell lines
224387pLenti_CMV_Puro_SFB_GNB1L_WD 6 deletionLenti plasmid for generating GNB1L _WD 6 domain depleted expressing stable cell lines
224388pLenti_CMV_Puro_SFB_GNB1L_WD 7 deletionLenti plasmid for generating GNB1L _WD 7 domain depleted expressing stable cell lines

Antibodies from Article