ABE-Mediated Cardiac Gene Silencing via Single AAVs Requires DNA Accessibility.
Liu Z, Yang L, Yang Y, Li J, Chen Z, Guo C, Guo Q, Li Q, Zhao D, Hu X, Gao F, Guo Y
Circ Res. 2025 Jan 16. doi: 10.1161/CIRCRESAHA.124.325611.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220117 | pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA1 | Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter |
| 220118 | pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA6 | Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter |
| 220119 | pAAV-EFS-TadA8e-Sauri-U6-Camk2d sgRNA7 | Expresses SauriABE8e by EFS promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 220120 | pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA1 | Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d start codon site by U6 promoter |
| 220122 | pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA6 | Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron6 5' splice site by U6 promoter |
| 220124 | pAAV-MYL2-TadA8e-Sauri-U6-Camk2d sgRNA7 | Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 220127 | pAAV-MYL2-HA-TadA8e-Sauri-U6-Camk2d sgRNA7 | Expresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadA |
| 220128 | pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN | Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA |
| 220129 | pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7 | Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 226678 | pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7 | Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 226915 | pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7 | Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 226916 | pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7 | Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter |
| 226921 | pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold | Encoding SauriABE8e and sgRNA cassette on a single AAV |
| 226933 | pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold | Encoding Nme2ABE8e and sgRNA cassette on a single AAV |
| 226935 | pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold | Encoding SaKKHABE8e and sgRNA cassette on a single AAV |