Validation of a heat-inducible Ixodes scapularis HSP70 promoter and developing a tick-specific 3xP3 promoter sequence in ISE6 cells.

Pham M, Hoffmann HH, Kurtti TJ, Chana R, Garcia-Cruz O, Aliabadi S, Gulia-Nuss M
bioRxiv [Preprint]. 2023 Nov 29:2023.11.29.569248. doi: 10.1101/2023.11.29.569248. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
220091	Is3xP3-mCherry	Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)
220137	pGL 4.79-mCherry	promoterless vector