Repair of CRISPR-guided RNA breaks enables site-specific RNA excision in human cells.

Nemudraia A, Nemudryi A, Wiedenheft B
Science. 2024 Apr 25:eadk5518. doi: 10.1126/science.adk5518. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
220044	pAN099	Mammalian expression of type III-A crRNA from Streptococcus thermophilus
220045	pAN102	Mammalian expression of type III-A crRNA and Cas6 crRNA-processing ribonuclease from Streptococcus thermophilus
220046	pAN052	Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags
220047	pAN053	Mammalian expression of type III-A crRNA from Streptococcus thermophilus within 5`UTR of mCherry transcript
220048	pAN007	Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.
220049	pAN008	Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope (positive control).
220050	pAN013	Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.
220051	pAN012	Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide (positive control).
220052	pAN056	Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).
220053	pAN057	NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).