Orthogonal Targeting of SAC1 to Mitochondria Implicates ORP2 as a Major Player in PM PI4P Turnover.

Doyle CP, Rectenwald A, Timple L, Hammond GRV
Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
220080	iRFP713-FRB-Fis1tail	FRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophore
220079	TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail	SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore
220078	TagBFP2-C1-SACM1LdeltaTMD-Fis1tail	SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore
220077	EGFP-1-SAC1deltaTMD-Fis1tail	SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore
220076	mCherry-C1-FKBPflex-ELL-CAAX	an extra long linker attached to a CAAX domain with an mCherry tag
220075	mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP	expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP
220074	mCherry-C1-ORP5deltaORDdeltaTMD-FKBP	expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.
220073	mCherry-C1-ORP5deltaTMD-FKBP	expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.
220072	NeonGreen-C1-ORP6	overexpresses ORP6 with a neonGreen fluorophore
220071	NeonGreen-C1-ORP5	overexpresses ORP5 with a neonGreen fluorophore