Orthogonal Targeting of SAC1 to Mitochondria Implicates ORP2 as a Major Player in PM PI4P Turnover.
Doyle CP, Rectenwald A, Timple L, Hammond GRV
Contact (Thousand Oaks). 2024 Feb 7;7:25152564241229272. doi: 10.1177/25152564241229272. eCollection 2024 Jan-Dec.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220069 | NeonGreen-C1-ORP2 | overexpresses ORP2 with a neonGreen fluorophore |
| 220070 | NeonGreen-C1-ORP3 | overexpresses ORP3 with a neonGreen Fluorophore |
| 220071 | NeonGreen-C1-ORP5 | overexpresses ORP5 with a neonGreen fluorophore |
| 220072 | NeonGreen-C1-ORP6 | overexpresses ORP6 with a neonGreen fluorophore |
| 220073 | mCherry-C1-ORP5deltaTMD-FKBP | expresses ORP5 with the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5. |
| 220074 | mCherry-C1-ORP5deltaORDdeltaTMD-FKBP | expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5. |
| 220075 | mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP | expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP |
| 220076 | mCherry-C1-FKBPflex-ELL-CAAX | an extra long linker attached to a CAAX domain with an mCherry tag |
| 220077 | EGFP-1-SAC1deltaTMD-Fis1tail | SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Green Fluorophore |
| 220078 | TagBFP2-C1-SACM1LdeltaTMD-Fis1tail | SAC1 with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore |
| 220079 | TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail | SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore |
| 220080 | iRFP713-FRB-Fis1tail | FRB targeted to the mitochondria with the tail of the mitochondrial outer membrane protein Fis1 with an iRFP fluorophore |