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Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates.

Huang HT, Lumpkin RJ, Tsai RW, Su S, Zhao X, Xiong Y, Chen J, Mageed N, Donovan KA, Fischer ES, Sellers WR
Nat Chem Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
220146pLX307-CRBN-3F-BirAExpresses CRBN-BirA for CRBN substrate identification by E-STUB
220147pLX307-VHL-3F-BirAExpresses VHL-BirA for VHL substrate identification by E-STUB
220148pLX307-VHL(Y98H)-3F-BirAExpresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB
220149pLX307-VHL(C162F)-3F-BirAExpresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB

Antibodies from Article