Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates.
Huang HT, Lumpkin RJ, Tsai RW, Su S, Zhao X, Xiong Y, Chen J, Mageed N, Donovan KA, Fischer ES, Sellers WR
Nat Chem Biol. 2024 Mar 21. doi: 10.1038/s41589-024-01590-9.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220145 | pRK5-A3-HA-ubiquitin | Expresses A3-Ub for E3 substrate identification by E-STUB |
| 220146 | pLX307-CRBN-3F-BirA | Expresses CRBN-BirA for CRBN substrate identification by E-STUB |
| 220147 | pLX307-VHL-3F-BirA | Expresses VHL-BirA for VHL substrate identification by E-STUB |
| 220148 | pLX307-VHL(Y98H)-3F-BirA | Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB |
| 220149 | pLX307-VHL(C162F)-3F-BirA | Expresses VHL(C162F)-BirA as a control for VHL substrate identification by E-STUB |