Precise kilobase-scale genomic insertions in mammalian cells using PASTE.
Fell CW, Schmitt-Ulms C, Tagliaferri DV, Gootenberg JS, Abudayyeh OO
Nat Protoc. 2024 Dec 15. doi: 10.1038/s41596-024-01090-z.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219859 | PASTE payload acceptor with attP site pDY2087 | Bxb1 payload vector with 5' attP |
| 220989 | pDY2259 hNOLC1 atgRNA Paired Guide 1 | hNOLC1 atgRNA |
| 220990 | pDY2260 hNOLC1 atgRNA Paired Guide 2 | hNOLC1 atgRNA |
| 220991 | pDY2261 hACTB atgRNA Paired Guide 1 | hACTB atgRNA |
| 220992 | pDY2262 hACTB atgRNA Paired Guide 2 | hACTB atgRNA |