Philipp Maass Lab: LncRNA CISTR-ACT regulates cell size in human and mouse

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
219819	pXR003_puroR	hU6-driven sgRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #109053)
219820	pXR004_puroR	hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)
219821	miniTurbo-dCasRx	EF1α-driven expression of miniTurbo fused to dCasRx with GFP (Adapted from plasmid #153209)
219822	dCas9-miniTurbo	EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)
219823	hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone	EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)
219825	CMV-dCas9_puroR	CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
219826	LncRNA overexpression EF1α-BbsI-SV40polyA_puroR	Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.
219828	LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP	Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.