Rapid, biochemical tagging of cellular activity history in vivo.
Zhang R, Anguiano M, Aarrestad IK, Lin S, Chandra J, Vadde SS, Olson DE, Kim CK
Nat Methods. 2024 Sep;21(9):1725-1735. doi: 10.1038/s41592-024-02375-7. Epub 2024 Aug 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219779 | pAAV-CMV-CaM-V5-sTb(N) | sTb(N) half of CaST for HEK cell expression |
| 219780 | pAAV-CMV-CD4-sTb(C)-M13-GFP | sTb(C) half CaST for HEK cell expression |
| 219781 | pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN | CaST-P2A for HEK cell expression |
| 219782 | pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN | CaST-IRES for HEK cell expression |
| 219783 | pAAV-Syn-CaM-V5-sTb(N) | sTb(N) half of CaST for neuron expression |
| 219784 | pAAV-Syn-CD4-sTb(C)-M13-GFP | sTb(C) half of CaST for neuron expression |
| 222742 | pAAV-Syn-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN | CaST-IRES for neuron expression |