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Addgene

Jennifer Nemhauser Lab: Nemhauser Lab Plasmids 2024

Unpublished

Plasmids from Article

ID Plasmid Purpose
219686L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
219687L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
219688L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
219689L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
219690L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
219691L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2Level 0 to construct history-dependent target T2PB
219693L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6Level 0 to construct history-dependent target T2PB
219695L0-T12 GG2-ER-tagBFP-tUBQ10-GG3Level 0 to construct history-dependent target
219696L0-T13 GG3-tRBCS-mCherry-NLS-GG4Level 0 to construct history-dependent target
219697L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1Level 0 to construct history-dependent target
219698L0-T15 BpiI-p35s-BpiILevel 0 to insert p35S promoter to history-dependent target
219702L1P137 - pAHP6-PhiC31-NLS-tUBQ1Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
219703L1P139 - pSPCH-PhiC31-tUBQ1Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase
219706L0I84 - GG0-pAHP6-GG1Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly.
219707L0I87-SPCHPromoter_GG0-1Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly.
219708L0I89-pMUTE_GG0-1Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly.
219709L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
219711L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.
219713L0I74-_tUBQ10_GG4-GG6Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly.
219714L0I75_tUBQ10_GG5-GG6Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.
219715L0I80_Bxb1mut(noBsaI)_GG1-GG4Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly.

Antibodies from Article