Jennifer Nemhauser Lab: Nemhauser Lab Plasmids 2024
Unpublished
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
219686 | L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen) | Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
219687 | L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen) | Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
219688 | L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen) | Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
219689 | L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen) | Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
219690 | L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen) | Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
219691 | L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2 | Level 0 to construct history-dependent target T2PB |
219693 | L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6 | Level 0 to construct history-dependent target T2PB |
219695 | L0-T12 GG2-ER-tagBFP-tUBQ10-GG3 | Level 0 to construct history-dependent target |
219696 | L0-T13 GG3-tRBCS-mCherry-NLS-GG4 | Level 0 to construct history-dependent target |
219697 | L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1 | Level 0 to construct history-dependent target |
219698 | L0-T15 BpiI-p35s-BpiI | Level 0 to insert p35S promoter to history-dependent target |
219702 | L1P137 - pAHP6-PhiC31-NLS-tUBQ1 | Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase |
219703 | L1P139 - pSPCH-PhiC31-tUBQ1 | Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase |
219706 | L0I84 - GG0-pAHP6-GG1 | Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
219707 | L0I87-SPCHPromoter_GG0-1 | Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
219708 | L0I89-pMUTE_GG0-1 | Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
219709 | L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4 | Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites. |
219711 | L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4 | Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites. |
219713 | L0I74-_tUBQ10_GG4-GG6 | Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly. |
219714 | L0I75_tUBQ10_GG5-GG6 | Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly. |
219715 | L0I80_Bxb1mut(noBsaI)_GG1-GG4 | Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly. |