Jennifer Nemhauser Lab: Nemhauser Lab Plasmids 2024
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219686 | L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen) | Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
| 219687 | L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen) | Level 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
| 219688 | L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen) | Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
| 219689 | L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen) | Level 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
| 219690 | L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen) | Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance). |
| 219691 | L0-T6 Circuit 1 GG1-Bxb1P-bbs1-PhiC31P-GG2 | Level 0 to construct history-dependent target T2PB |
| 219692 | L0-T7 Circuit 2 GG1-PhiC31P-bbs1-BxB1P-GG2 | Level 0 to construct history-dependent target T2BP |
| 219693 | L0-T10 Circuit 1 GG4-Bxb1B-PhiC31B-GG6 | Level 0 to construct history-dependent target T2PB |
| 219694 | L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6 | Level 0 to construct history-dependent target T2BP |
| 219695 | L0-T12 GG2-ER-tagBFP-tUBQ10-GG3 | Level 0 to construct history-dependent target |
| 219696 | L0-T13 GG3-tRBCS-mCherry-NLS-GG4 | Level 0 to construct history-dependent target |
| 219697 | L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1 | Level 0 to construct history-dependent target |
| 219698 | L0-T15 BpiI-p35s-BpiI | Level 0 to insert p35S promoter to history-dependent target |
| 219699 | L1PB1 - pSPCH--PhiC31-tUBQ1 - pMUTE--Bxb1-tUBQ10 | Level 1 PhiC31 and Bxb1 integrase construct for tracking of stomata development: pSPCH promoter for PhiC31 and pMUTE promoter for Bxb1, no tag |
| 219701 | L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10 | Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1 |
| 219702 | L1P137 - pAHP6-PhiC31-NLS-tUBQ1 | Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase |
| 219703 | L1P139 - pSPCH-PhiC31-tUBQ1 | Level 1 pSPCH-PhiC31-tUBQ1, for early stage of stomata development expression of PhiC31 integrase |
| 219704 | L1B39 - pMUTE-Bxb1-tUBQ10 | Level 1 pMUTE-Bxb1-tUBQ10, for stomata specific expression of Bxb1 integrase |
| 219705 | L1B42-pGATA23-Bxb1-SAUR-tUBQ10 | Level 1 pGATA23-Bxb1-SAUR-tUBQ10, for lateral root specific expression of Bxb1 integrase with SAUR tag |
| 219706 | L0I84 - GG0-pAHP6-GG1 | Level 0 pAHP6 promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
| 219707 | L0I87-SPCHPromoter_GG0-1 | Level 0 pSPCH promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
| 219708 | L0I89-pMUTE_GG0-1 | Level 0 pMUTE promoter, GG0 and GG1 BsaI sites for golden gate assembly. |
| 219709 | L0I90 - GG0-pSPCH-PhiC31-tUBQ1-GG4 | Level 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites. |
| 219711 | L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4 | Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites. |
| 219712 | L0I101- GG4-pGATA23-Bxb1-tUBQ10-GG6 | Level 0 pGATA23-Bxb1-tUBQ10 construct for assembly of two integrase constructs, GG4 and GG6 BsaI sites. |
| 219713 | L0I74-_tUBQ10_GG4-GG6 | Level 0 tUBQ10 terminator, GG4 and GG6 BsaI sites for golden gate assembly. |
| 219714 | L0I75_tUBQ10_GG5-GG6 | Level 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly. |
| 219715 | L0I80_Bxb1mut(noBsaI)_GG1-GG4 | Level 0 Bxb1 integrase with mutated BsaI sites, GG1 and GG4 BsaI sites for golden gate assembly. |