Seamless knockins in Drosophila via CRISPR-triggered single-strand annealing.
Aguilar G, Bauer M, Vigano MA, Schnider ST, Brugger L, Jimenez-Jimenez C, Guerrero I, Affolter M
Dev Cell. 2024 Oct 7;59(19):2672-2686.e5. doi: 10.1016/j.devcel.2024.06.004. Epub 2024 Jul 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219639 | pSEED-sfGFP | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method |
| 219640 | pSEED-mScarlet3 | Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method |
| 219641 | pSEED-mScarlet3:ALFA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method |
| 219642 | pSEED-sfGFP:ALFA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method |
| 219643 | pSEED-mGreenLantern | Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method |
| 219644 | pSEED-mGreenLantern:ALFA | Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method |
| 219645 | pSEED-Dendra2 | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method |
| 219646 | pSEED-ALFA:HA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in ALFA and HA tags using the SEED/Harvest method |
| 219647 | pSEED-OLLAS:HA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method |
| 219648 | pSEED-Moontag:HA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method |
| 219656 | pSEED-EYFP:HA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method |
| 219657 | pSEED-mCherry | Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method |
| 219658 | pSEED | Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI) |
| 219660 | pSEED-LoxP-sfGFP:ALFA | Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED. |
| 219661 | pUASTattB-dALFANb(5mut)-mGreenLantern | Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLantern |
| 219662 | pUASTattB-dALFANb(3mut)-mGreenLantern | Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern |
| 219663 | pUASTattB-degradALFA | Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb. |