Assessing and engineering the IscB-omegaRNA system for programmed genome editing.
Yan H, Tan X, Zou S, Sun Y, Ke A, Tang W
Nat Chem Biol. 2024 Jul 8. doi: 10.1038/s41589-024-01669-3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219634 | OgeuIscB ωRNA-v13 | human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning |
| 219636 | enOgeuIscB | CMV-SV40 NLS-enOgeuIscB-nucleoplasmin NLS |
| 220939 | enOgeuIscB with ωRNA-v2 | CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing |
| 220940 | enOgeuIscB with ωRNA-v13 | CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing |
| 220958 | OgeuIscB ωRNA-v2 | human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing |