Programmed RNA editing with an evolved bacterial adenosine deaminase.

Yan H, Tang W
Nat Chem Biol. 2024 Jul 5. doi: 10.1038/s41589-024-01661-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
219655	CasRx gRNA targeting EZH2	hU6-driven expression of CasRx guide RNA targeting EZH2. 5' processed DR followed by EZH2 guide.
219547	hfDECOR-V106G	Expression of CMV-TadA8e-V106G-NLS-dCas13d-NLS in mammalian cells (hfDECOR-V106G expression)
219546	hfDECOR-F148A	Expression of CMV-TadA8e-F148A-NLS-dCas13d-NLS in mammalian cells (hfDECOR-F148A expression)
219545	DECOR	Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)