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Complementation of a human disease phenotype in vitro by intercellular mRNA transfer

Haimovich G, Dasgupta S, Ravi AG, Gerst JE
bioRxiv 2024.11.06.622258 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183726pHAGE-RFP-SKLFor expression of RFP targeted to peroxisomes
183727pHAGE-EGFP-SKLFor exrpression of GFP targeted to peroxisomes
183728pHAGE-mCardinal-SKLFor exrpression of mCardinal targeted to peroxisomes
183729pHAGE-mNeptune2-SKLFor exrpression of mNeptune2 targeted to peroxisomes
183730pHAGE-PEX6-TomatoExpression of human PEX6 fused to Tomato, and PEX6 3'UTR
183731pHAGE-7xHA-PEX6-TomatoExpression of human 7xHA- PEX6 fused to Tomato, and PEX6 3'UTR
219536pLenti-Cre-MS2_UbC-GFP (Cre_GFP)a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor
219537pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)a. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotor

Antibodies from Article