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A RAB7A phosphoswitch coordinates Rubicon Homology protein regulation of Parkin-dependent mitophagy.

Tudorica DA, Basak B, Puerta Cordova AS, Khuu G, Rose K, Lazarou M, Holzbaur ELF, Hurley JH
J Cell Biol. 2024 Jul 1;223(7):e202309015. doi: 10.1083/jcb.202309015. Epub 2024 May 10. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
216698pmCherryC1-PacerMammalian expression of Pacer
216706pMAL-c5e-Rubicon RHBacterial protein expression of Rubicon RH domain
216707pMAL-c5e-Rubicon RH N821K, T911R, K916RBacterial protein expression of Rubicon RH domain mutant
216709pCAG-TSF-TBK1Mammalian expression of TBK1
219440pHTN-LC3BExpresses HaloTag-LC3B, an autophagosome marker
219441pCIG2-IRES-Lamp1-mNeon GreenExpresses Lamp1 fused to mNeon Green

Antibodies from Article