Efficient scar-free knock-ins of several kilobases in plants by engineered CRISPR/Cas endonucleases.
Schreiber T, Prange A, Schafer P, Iwen T, Grutzner R, Marillonnet S, Lepage A, Javelle M, Paul W, Tissier A
Mol Plant. 2024 Mar 22:S1674-2052(24)00086-8. doi: 10.1016/j.molp.2024.03.013.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 219428 | pAGT6471 | Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG) |
| 219429 | pAGT9050 | PapE Exonuclease fused to linker 4LF2 as N-terminal tag module (AATG_PapE-4*LF2_AGGT) |
| 219430 | pAGT9054 | Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS |
| 219431 | pAGT9569 | Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS |