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Leveraging a self-cleaving peptide for tailored control in proximity labeling proteomics.

Delhaye L, Moschonas GD, Fijalkowska D, Verhee A, De Sutter D, Van de Steene T, De Meyer M, Grzesik H, Van Moortel L, De Bosscher K, Jacobs T, Eyckerman S
Cell Rep Methods. 2024 Jul 15;4(7):100818. doi: 10.1016/j.crmeth.2024.100818. Epub 2024 Jul 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

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Plasmids from Article

ID Plasmid Purpose
218890pLV-W-ccdB-Zgolden gate cloning
218891pEN-W-A-ccdB-G-Xgolden gate cloning
218892pEN-X-A-ccdB-G-Zgolden gate cloning
218893pPB-W-ccdB-Zgolden gate cloning
218894pGGA-pTRE-Bgolden gate cloning
218895pGGB-Kozak-ATG-Cgolden gate cloning
218896pGGB-V5-TurboID-T2A-Cgolden gate cloning
218897pGGB-V5-TurboID-MUTT2A-Cgolden gate cloning
218898pGGD-T2A-Egolden gate cloning
218899pGGD_MUTT2A_Egolden gate cloning
218900pGGE-V5_TurboID-Fgolden gate cloning
218901pGGF-WPRE-Ggolden gate cloning
218902pGGF-bGH polyA-Ggolden gate cloning
218903pEN-X-mPGK-BlastR-SV40 polyA-Zgolden gate cloning
218904pEN-X-hPGK-PuroR-Zgolden gate cloning
218905pEN-X-hPGK-HygroR-Zgolden gate cloning
218906pEN-X-hPGK-NeoR-Zgolden gate cloning
218907pEN-X-hPGK-BlastR-Zgolden gate cloning
218908pEN-X-mPGK-PuroR-SV40 polyA-Zgolden gate cloning
218909pEN-X-mPGK-NeoR-SV40 polyA-Zgolden gate cloning
218910pPB-TRE-V5-TurboID-linker-T2A-GR-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218911pPB-TRE-V5-TurboID-linker-MUTT2A-GR-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218912pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218913pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NCAP-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218914pPB-TRE-V5-TurboID-T2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218915pPB-TRE-V5-TurboID-MUTT2A-SARS-CoV2-NSP7-bGH polyA > mPGK-BlastR-SV40 polyAPiggyBac transposition
218916pLV-EF1a-NLS-msfGFP-T2A-mCherry-NES> mPGK-PuroRLentiviral construct expressing msfGFP and nuclear mCherry proteins.

Antibodies from Article