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An optogenetic method for the controlled release of single molecules.

Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H
Nat Methods. 2024 Mar 8. doi: 10.1038/s41592-024-02204-x. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
219674pTMEM-PhoCl-InsertCaging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarlet
219675pRER-PhoCl-InsertCaging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2
219676pInsert-PhoCl-RERCaging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

Antibodies from Article