Omar Akbari Lab: CRISPR-Cas transgene repressible element
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 220957 | 1203M-CMV-dDiCas7-11-bGHpolyA | Deactivated DiCas7-11 effector with CMV promoter/enhancer |
| 220959 | 1203O-CMV-DiCas7-11-Δ7.1-bGHpolyA | DiCas7-11 effector with Cas7.1 domain deletion mutation |
| 220960 | 1203P-CMV-Cas7.1-bGHpolyA | Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector) |
| 220961 | 1203W-CMV-Cas7.1-7.2-bGHpolyA | Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and Cas7.2 domains from DiCas7-11, deactivated (truncated from DiCas7-11 effector) |
| 220962 | 1203R-CMV-3'dmDR-EGFP-bGHpolyA | EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 3' end |
| 221448 | 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR | Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3 |
| 221449 | 1203AE-CMV-Cas7.1-7.2-H43A-mutant | Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, H to A mutation at amino acid 43 in Cas7.1 |
| 221450 | 1203AF-CMV-Cas7.1-7.2-Y55A-mutant | Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, Y to A mutation at amino acid 55 in Cas7.1 |
| 221451 | 1203AG-CMV-Cas7.1-7.2-N152A-mutant | Effector expresses Cas7.1, Linker1, Cas11, Linker 2, and deactivated Cas7.2 domains from DiCas7-11, N to A mutation at amino acid 152 in Cas7.1 |
| 221452 | 1203A-CMV-5’DR-EGFP-bGHpolyA | EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end |
| 221453 | 1203B-CMV-3’DR-EGFP-bGHpolyA | EGFP reporter construct with single unprocessed direct repeat (35nt) at 3' end |
| 221454 | 1203C-CMV-5’+3'DR-EGFP-bGHpolyA | EGFP reporter construct with single unprocessed direct repeat (35nt) at 5' end and 3' end |
| 221534 | 1203D-5'dDR-mCh(22)-GFP-bGHpolyA | EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' end, 22nt mCherry-targeting spacer in between DRs |
| 221535 | 1203E-3'dDR-mCh(22)-GFP-bGHpolyA | EGFP reporter construct with double unprocessed direct repeat (35nt) at 3' end, 22nt mCherry-targeting spacer in between DRs |
| 221536 | 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA | EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs |
| 221537 | 1203N-CMV-dDiCas7-11-NLS-bGHpolyA | Deactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domain |
| 221538 | 1203Q-5'dmDR-NT(22)-GFP-bGHpolyA | EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' end |
| 221539 | 1203S-5'+3’dmDR-NT(22)-GFP-bGHpolyA | EGFP reporter construct with double processed direct repeats (15nt) and a non-target spacer in between at 5' and 3' end |
| 221540 | 1203T-CMV-Cas7.1-NLS-bGHpolyA | Effector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domain |
| 221541 | 1203AB-CMV-Cas7.1-H43A-mutant | Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43 |
| 221542 | 1203AC-CMV-Cas7.1-Y55A-mutant | Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55 |
| 221543 | 1203AD-CMV-Cas7.1-N152A-mutant | ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152 |