Continuous evolution of compact protein degradation tags regulated by selective molecular glues.
Mercer JAM, DeCarlo SJ, Roy Burman SS, Sreekanth V, Nelson AT, Hunkeler M, Chen PJ, Donovan KA, Kokkonda P, Tiwari PK, Shoba VM, Deb A, Choudhary A, Fischer ES, Liu DR
Science. 2024 Mar 15;383(6688):eadk4422. doi: 10.1126/science.adk4422. Epub 2024 Mar 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218789 | pSSRB070_pAC8-hsCRBN | Insect cell expression vector for FLAG-TEV-Spy-hsCRBN |
| 218790 | pSSRB069_pAC8-hsDDB1dB | Insect cell expression vector for 6xHis-TEV-hsDDB1∆B |
| 218791 | pSSRB274_pAC8-eGFP-hsCRBN | Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN |
| 224113 | pSDC803_IKZF1_mutant | Insect cell expression vector for IKZF1 mutant for CRBN cryoEM pre-blotting |