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A Robust Platform for Unnatural Amino Acid Mutagenesis in E. coli Using the Bacterial Tryptophanyl-tRNA synthetase/tRNA pair.

Ficaretta ED, Wrobel CJJ, Roy SJS, Erickson SB, Italia JS, Chatterjee A
J Mol Biol. 2022 Apr 30;434(8):167304. doi: 10.1016/j.jmb.2021.167304. Epub 2021 Oct 13. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
218769ATMW-BL21E. coli strain, genotype: pUltraG ScW40CCA BL21 trpT::gentR trpS::zeoR

Antibodies from Article