Observing isolated synaptic vesicle association and fusion ex vivo.
Leitz J, Wang C, Esquivies L, Peters JJ, Gopal N, Pfuetzner RA, Wang AL, Brunger AT
Nat Protoc. 2024 Jul 2. doi: 10.1038/s41596-024-01014-x.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 218673 | pDC180 | Expresses rat ⍺SNAP in bacteria |
| 218674 | C1C2CMUNC2C | Insect cell expression of a rat MUNC13-1 fragment containing domains C1C2BMUNC2C |
| 218676 | CpX-1 | Expresses complexin-1 in bacteria |
| 218677 | vGlut1 peptide- GST | Expresses a GST fusion to vGlut peptide for immunoisolation of glutamatergic vesicles |
| 218888 | MUNC18a | Expresses rat MUNC18a in bacteria |
| 218889 | MUNC13-1 MUN | Expresses the MUN domain from MUNC13-1 in bacteria |